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Anti-6 His Antibody, FITC conjugate (18812F)

Host Quantity Applications Species Reactivity Data Sheet  
Goat 100ug IHC,ICC,IF 6 His
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SKU: 18812F Categories: , ,
Overview
Product Name Anti-6 His Antibody, FITC conjugate (18812F)
Description Anti-6 His FITC Goat Polyclonal Antibody
Target 6 His
Species Reactivity 6 His
Applications IHC,ICC,IF
Host Goat
Clonality Polyclonal
Isotype Whole IgG
Conjugate FITC
Immunogen 6-His (HHHHHH) conjugated to KLH.
Source Escherichia coli
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Specificity Information
Specificity Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Target ID 6 His
Research Areas Epitope Tags
Background A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunofluorescence: use at a dilution of 1:100-1:400.

These are recommended dilutions.

End users should determine optimal dilutions for their applications.
References & Data Sheet