Anti-VSV-G Antibody, HRP conjugate (18874P)

$3,142.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 1mg ELISA,ICC,IHC,WB Vsv-G
SKU: 18874P Categories: , ,
Overview
Product Name Anti-VSV-G Antibody, HRP conjugate (18874P)
Description Anti-VSV-G HRP Goat Polyclonal Antibody
Target VSV-G
Species Reactivity Vsv-G
Applications ELISA,ICC,IHC,WB
Host Goat
Clonality Polyclonal
Conjugate HRP
Conjugate Ratio HRP, Molar enzyme/antibody ratio = 4:1.
Immunogen Synthetic peptide YTDIEMNRLGK conjugated to KLH.
Source Escherichia coli
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.05% Pro-Clean 400
Buffer Protein Stabilizer 0.2% Bovine Serum Albumin
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID VSV-G
Research Areas Epitope Tags
Background The Vesicular Stomatitis Virus Glycoprotein (VSV-G) epitope tag is represented by the sequence YTDIEMNRLGK. This tag is often included in recombinant proteins to facilitate identification and isolation of those proteins.
Application Images
Description Detection of VSV-G-tagged fusion protein in 200, 100, and 50ng of E. coli lysate with #18874P diluted 1:25,000.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:30,000.

Immunocytochemistry: use at a dilution of 1:200-1:500

ELISA: use at a dilution of 1:10,000- 1:100,000

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet