Anti-Protocadherin γ (pan) Antibody (56562)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF Human, Mouse, Rat
SKU: 56562 Categories: , ,
Overview
Product Name Anti-Protocadherin γ (pan) Antibody (56562)
Description Anti-Protocadherin γ (pan) Mouse Monoclonal Antibody
Target Protocadherin γ (pan)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S159-5
Isotype IgG1
Immunogen Fusion protein corresponding to aa 808-931 (C-terminal cytoplasmic constant domain) of mouse PCDHGA1. This sequence is present in all 22 PCDHG proteins.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat PCDHG-A, B, and C proteins
Target Name Protocadherin γ A1
Target ID Protocadherin γ (pan)
Uniprot ID Q91XZ0
Gene Name Pcdhga1
Research Areas Neuroscience
Background The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. The gamma gene cluster includes 22 genes divided into 3 subfamilies: subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region (which includes 6 cadherin ectodomains and a trans-membrane region). The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins are believed to play a critical role in the establishment and function of specific cell-cell connections in the brain.
Application Images
Description Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone S159-5 (56562). Load: 10 µg. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody (56562) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone S159-5 (56562). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody (56562) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Protocadherin Gamma (pan) Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone S159-5 (56562). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody (56562) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Nucleus . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Protocadherin Gamma (pan) Antibody. (D) Composite.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL. A band of ~100kDa is detected.

Immunofluorescence: use at 10ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their application.
References & Data Sheet