Anti-Nav1.8 Na+ Channel Antibody (11523)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,AM Human, Mouse, Rat
SKU: 11523 Categories: , ,
Overview
Product Name Anti-Nav1.8 Na+ Channel Antibody (11523)
Description Anti-Nav1.8 Na+ Channel Mouse Monoclonal Antibody
Target Nav1.8 Na+ Channel
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF,AM
Host Mouse
Clonality Monoclonal
Clone ID S134-12
Isotype IgG2a
Immunogen Fusion protein, aa 1724-1956 (cytoplasmic C-terminus) of rat Nav1.8 (accession no. Q62968).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Nav1.8. It does not cross-react with other Nav channels.
Target Name Sodium channel protein type 10 subunitα
Target ID Nav1.8 Na+ Channel
Uniprot ID Q62968
Alternative Names Peripheral nerve sodium channel 3, PN3, Sensory neuron sodium channel, Sodium channel protein type X subunitα, Voltage-gated sodium channel subunitα Nav1.8
Gene Name Scn10a
Accession Number NP_058943.1
Sequence Location Cell membrane
Biological Function Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms. {PubMed:12514212, PubMed:8538791, PubMed:8626372}.
Research Areas Ion Channels
Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Nav1.8 is a voltage-gated sodium channel that plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Since Nav1.8 is not present in cardiac muscle or neurons in the CNS, blockers of Nav1.8 will not directly affect those cells and can have less side effects than current pain medications.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134 (11523). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody (11523) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic staining and some dull nuclear staining.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134 (11523). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody (11523) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav1.8 Antibody (D) Composite.
Description Immunohistochemistry analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134 (11523). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody (11523) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Heavy filaggrin-like staining, lower epidermal cells have some staining.
Description Western Blot analysis of Monkey COS transient cell lysate showing detection of Nav1.8 protein using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134 (11523). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody (11523) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/mL. A band of ~220kDa is detected.Immunohistochemistry and

Immunocytochemistry: use at 0.1-1ug/mL

Immunofluorescence: use at 1-10ug/mL

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Rat dorsal root ganglia or lysate of COS cells transiently expressing Nav1.8.
References & Data Sheet