Anti-MEF2a (phospho-Thr312) Antibody (43039)
$366.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Rabbit | 100ul | IHC,IF | Human, Mouse, Rat |  |
Overview
Product Name Anti-MEF2a (phospho-Thr312) Antibody (43039)
Description Anti-MEF2a (phospho-Thr312) Rabbit Polyclonal Antibody
Target MEF2a (phospho-Thr312)
Species Reactivity Human, Mouse, Rat
Applications IHC,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of threonine 312 (L-A-T(p)-P-V) derived from human MEF2a and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat MEF2a only when phosphorylated at threonine 312.
Target Name Myocyte-specific enhancer factor 2A
Target ID MEF2a (phospho-Thr312)
Uniprot ID Q02078
Gene Name MEF2A
Post Translational Modification MEF2a (phospho-Thr312)
Target Phospho-thr312
Sequence Location Nucleus
Biological Function Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter. {PubMed:11904443, PubMed:12691662, PubMed:15834131, PubMed:16371476, PubMed:16484498, PubMed:16563226, PubMed:21468593, PubMed:9858528}.
Research Areas Phosphospecific Antibodies
Background The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression including the DNA binding regulatory protein myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many muscle-specific genes. The MEF2 genes are members of the MADS gene family that also includes several homeotic genes and other transcription factors all of which share a conserved DNA-binding domain.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions
Immunohistochemistry: use at dilution of 1:50- 1:100.
Immunofluorescence: use at dilution of 1:100- 1:200.
These are recommended working dilutions.
End user should determine optimal dilutions for their applications.
Immunohistochemistry: use at dilution of 1:50- 1:100.
Immunofluorescence: use at dilution of 1:100- 1:200.
These are recommended working dilutions.
End user should determine optimal dilutions for their applications.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet