Anti-HGAL Antibody (34044)

$372.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC Human
SKU: 34044 Categories: , ,
Overview
Product Name Anti-HGAL Antibody (34044)
Description Anti-HGAL Mouse Monoclonal Antibody
Target HGAL
Species Reactivity Human
Applications WB,IHC
Host Mouse
Clonality Monoclonal
Clone ID 1H1-A7
Isotype IgG2a
Immunogen GST-HGAL fusion protein (Accession no. AF521911)
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human HGAL.
Target Name Germinal center-associated signaling and motility protein
Target ID HGAL
Uniprot ID Q8N6F7
Alternative Names Germinal center B-cell-expressed transcript 2 protein, Germinal center-associated lymphoma protein, hGAL
Gene Name GCSAM
Sequence Location Cytoplasm. Cell membrane. Note=It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6.
Biological Function Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. {PubMed:17823310, PubMed:20844236, PubMed:23299888}.
Research Areas Cancer research
Background The human germinal-center-associated lymphoma (HGAL) gene was originally identified from an expressed sequence tag that was associated with improved survival in patients with diffuse large B-cell lymphoma (DLBCL). Cloning and characterization of HGAL indicated that it is induced by IL-4. HGAL is a cytoplasmic protein and has been demonstrated in follicular lymphoma, Burkitt lymphoma, lymphocyte-predominant Hodgkin lymphoma, and a subset of DLBCL. HGAL expression is an important predictor of overall survival in patients with germinal center- derived tumors. HGAL protein is also found in germinal centers of normal tonsils and lymph nodes.
Application Images
Description Immunohistochemistry: use at 1-10ug/ml on paraffin-embedded tissues with heat-induced antigen retrieval in citrate buffer (10mM, pH 6.0, 10 minutes).
Description HGAL mRNA and protein expression in lysates of indicated cell lines. Actin served as a loading control.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry: use at 1-10ug/ml on paraffin-embedded tissues with heat-induced antigen retrieval in citrate buffer (10mM, pH 6.0, 10 minutes).

Immunoblotting: use at 1-10ug/mlHGAL mRNA and protein expression in lysates of indicated cell lines. Actin served as a loading control.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet
References Fouad-Younes S et al. 2010 Am J Surg Pathol 34: 1266-1276.
Natkunam Y et al. 2006 Blood 109: 298-305.
Natkunam Y et al. 2005 Blood 105: 3979-3986.
Lossos IS et al. 2003 Blood 101: 433-440.