Anti-DNA Ligase III Antibody (56250)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,IP,Blocking,PLA Human, Mouse, Chicken
SKU: 56250 Categories: , ,
Overview
Product Name Anti-DNA Ligase III Antibody (56250)
Description Anti-DNA Ligase III Mouse Monoclonal Antibody
Target DNA Ligase III
Species Reactivity Human, Mouse, Chicken
Applications WB,ICC/IF,IP,Blocking,PLA
Host Mouse
Clonality Monoclonal
Clone ID 1F3
Isotype IgG1
Immunogen Full-length recombinant human DNA Ligase III-b.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Hybridoma culture supernatant
Buffer Formulation Hybridoma culture supernatant
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and chicken DNA Ligase III. Cellular localization is nuclear and mitochondrial.
Target Name DNA ligase 3
Target ID DNA Ligase III
Uniprot ID P49916
Alternative Names EC 6.5.1.1, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3
Gene Name LIG3
Sequence Location [Isoform 1]: Mitochondrion
Biological Function Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627). {PubMed:10207110, PubMed:24674627}.
Research Areas Cancer research
Background There are four distinct ligase proteins found in mammalian cells. These DNA ligases are required for joining DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III is a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed, and complexes with XRCC-1 and a 97kDa (§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in base excision repair pathway.
Application Images
Description DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (56250) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description DNA ligase III detects DNA ligase III antibody [1F3] protein by western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
7.5% SDS-PAGE
DNA ligase III (56250) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Additional Information
Handling
Storage This antibody is stable for at least one year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-2ug/mL. Predicted molecular weight ~100kDa.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet