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Anti-CD4 Cytoplasmic Tail Antibody (18714)

$364.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug ELISA,IF Human
SKU: 18714 Categories: , ,
Overview
Product Name Anti-CD4 Cytoplasmic Tail Antibody (18714)
Description Anti-CD4 Cytoplasmic Tail Mouse Monoclonal Antibody
Target CD4 Cytoplasmic Tail
Species Reactivity Human
Applications ELISA,IF
Host Mouse
Clonality Monoclonal
Clone ID 8A5
Isotype IgM
Immunogen Synthetic peptide corresponding to aa sequence RFLPADQMNVADPQPLASCS at the post 3'-UGA codon region of CD4.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification PEG Purified
Specificity Information
Specificity This antibody recognizes the post 3'-UGA codon region of human CD4.
Target Name T-cell surface glycoprotein CD4
Target ID CD4 Cytoplasmic Tail
Uniprot ID P01730
Alternative Names T-cell surface antigen T4/Leu-3, CD antigen CD4
Gene Name CD4
Accession Number NP_000607
Sequence Location Cell membrane
Biological Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. {PubMed:16951326, PubMed:24942581, PubMed:2823150, PubMed:7604010}.; (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:2214026, PubMed:16331979, PubMed:9641677, PubMed:12089508). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607). {PubMed:12089508, PubMed:16331979, PubMed:17346169, PubMed:2214026, PubMed:7909607, PubMed:9641677}.
Research Areas Cell adhesion
Background CD4 is a co-receptor of the T-cell receptor (TCR) and assists the TCR in communicating with antigen-presenting cells. The short cytoplasmic/intracellular tail of CD4 contains a special sequence of amino acids that allow it to recruit and interact with the tyrosine kinase Lck (lymphocyte-specific protein tyrosine kinase). When the TCR and CD4 bind to distinct regions of an antigen-presenting MHCII molecule, tyrosine kinase Lck bound to the cytoplasmic tail of CD4 tyrosine-phosphorylates immunoreceptor tyrosine activation motifs (ITAM) on the cytoplasmic domains of CD3 to amplify the signal generated by the TCR. Many functions have been assigned to the cytoplasmic tail of CD4 including anti-viral regulation (down/up regulation of CD4), T-cell maturation, activation and differentiation, and Lck signal transmission.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: use at 10-20ug/ml. Optimized with 25ug cell lysate (Jurkat, H9, HeLa) on the solid phase.

Immunofluorescence: use at 5-10ug/ml. Optimized with CEM cells adhered to glass slides with poly-L-lysine and fixed in paraformaldehyde.
References & Data Sheet