Anti-Angiotensin Converting Enzyme 2 (ACE2) (19503)

$551.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human, Mouse
SKU: 19503 Categories: , ,
Overview
Product Name Anti-Angiotensin Converting Enzyme 2 (ACE2) (19503)
Description Anti-Angiotensin Converting Enzyme 2 (ACE2) Rabbit Polyclonal Antibody
Target Angiotensin Converting Enzyme 2 (ACE2)
Species Reactivity Human, Mouse
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids near the center of human ACE2 protein.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, 0.02% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.02% Sodium Azide
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity Human and mouse ACE2. Does not cross-react with ACE1.
Target Name Angiotensin-converting enzyme 2
Target ID Angiotensin Converting Enzyme 2 (ACE2)
Uniprot ID Q9BYF1
Gene Name ACE2
Gene ID 300335
Sequence Location [Processed angiotensin-converting enzyme 2]: Secreted, Cytoplasm, Cell projection, cilium, Apical cell membrane
Biological Function Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). {PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:18424768, PubMed:19021774, PubMed:19185582, PubMed:27217402}.; [Isoform 2]: Non-functional as a carboxypeptidase. {PubMed:33077916}.; (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. {PubMed:14647384, PubMed:15452268, PubMed:15791205, PubMed:15897467, PubMed:19901337, PubMed:24227843, PubMed:32142651, PubMed:32155444, PubMed:32221306, PubMed:32225175, PubMed:33000221, PubMed:33082294, PubMed:33432067}.; [Isoform 2]: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2. {PubMed:33077916, PubMed:33432184}.
Application Images
Description Immunoblotting: use at 0.5-2ug/ml. A band of ~92kDa is detected. Detection of ACE2 in human kidney lysate with #19503 at (A) 0.5, (B)1, and (C) 2ug/ml.
Description IHC: use at 2ug/ml. Detection of ACE2 in paraffin-embedded human kidney with #19503 at 2ug/ml.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions IHC: use at 2ug/ml.
References & Data Sheet