VGlut3 Transporter Monoclonal Antibody
Catalog No.: 56521 (clone S34-34)
Size: 100ug in PBS (1mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
The ATP-dependent, chloride-sensitive vesicular glutamate transporters include VGlut1, VGlut2, and VGlut3. VGlut3 is a multipass membrane protein restricted to synaptic vesicles of glutamatergic neurons. It is specifically expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord, and thalamus.
Antigen: Fusion protein corresponding to aa 546-588 (cytoplasmic C-terminus) of rat VGlut3 (accession no. NP_74947.1).
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human, mouse, and rat VGlut3. It does not cross-react with VGlut1 or VGlut2.
Immunoblotting: use at 1-2ug/ml. A band of ~65kDa is detected.
Immunohistochemistry: use at 1-5ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Positive control: CV-1 fibroblasts transfected with VGlut3.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.