VGlut1 Transporter Monoclonal Antibody
Catalog No.: 56514 (clone S28-9)
Size: 100ug in PBS (1mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
VGlut1 is expressed in a subset of glutamate neurons where it transports glutamate into native synaptic vesicles from the brain. Glutamate transport by VGlut1 is saturated with a K(m) of approximately 2mM. Whereas plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, VGlut1 recognizes only glutamate.
Antigen: Fusion protein corresponding to aa 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession no. NP_446311).
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human, mouse, and rat VGlut1. It does not cross-react with VGlut2.
Immunoblotting: use at 1-2ug/ml. A band of ~52kDa is detected.
Immunohistochemistry: use at 1-5ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Positive control: rat brain lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.