Anti-VDAC1 Antibody (56564)

$457.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF Human, Mouse, Rat
SKU: 56564 Categories: , ,
Overview
Product Name Anti-VDAC1 Antibody (56564)
Description Anti-VDAC1 Mouse Monoclonal Antibody
Target VDAC1
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S152B-23
Isotype IgG2a
Immunogen Fusion protein corresponding to aa 1-283 (full-length) of human VDAC1. This sequence is 98
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse and rat VDAC1. It does not cross-react with VDAC2 or VDAC3 (based on evaluation in knock-out mice).
Target Name Voltage-dependent anion-selective channel protein 1
Target ID VDAC1
Uniprot ID P21796
Alternative Names VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM
Gene Name VDAC1
Sequence Location Mitochondrion outer membrane, Cell membrane, Membrane raft, Multi-pass membrane protein
Biological Function Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterol cholesterol (PubMed:31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). May mediate ATP export from cells (PubMed:30061676). {PubMed:11845315, PubMed:15033708, PubMed:18755977, PubMed:20230784, PubMed:25296756, PubMed:30061676, PubMed:31015432, PubMed:32047033, PubMed:8420959}.
Research Areas Neuroscience
Background VDAC1 (also known as Voltage-dependent anion-selective channel protein 1 and outer mitochondrial membrane protein porin 1) is the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC1 forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation, and apoptosis. VDAC1 may participate in the formation of the permeability transition pore complex (PTPC) which is responsible for the release of mitochondrial products that trigger apoptosis.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (56564). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (56564) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VDAC1 Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (56564). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (56564) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Outer Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VDAC1 Antibody. (D) Composite.
Description Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (56564). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (56564) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5ug/mL. A band of ~30kDa is detected.

Immunofluorescence: use at 10ug/mL

These are recommended concentrations.

Endusers should determine optimal concentrations for their application.
References & Data Sheet