Vanillylmandelic Acid ELISA Kit (ECP7973)
$748.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name Vanillylmandelic Acid ELISA Kit (ECP7973)
Description Vanillylmandelic Acid ELISA Kit (ECP7973) is a Competitive Inhibition ELISA for the quantitiation of Vanillylmandelic Acid in a variety of aqueous sample types.
Target Vanillylmandelic Acid
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 198.17 g·mol−1
Background Minimum Level of Detection: 43.7 ng/mL
Dynamic range of quantitation: 123.5-10,000 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 123.5-10,000 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Vanillylmandelic Acid Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Vanillylmandelic Acid Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 x 120uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| TMB Substrate | 1 x 9mL |
| Stop Solution | 1 x 6mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Vanillylmandelic Acid
Alternative Names dl-4-Hydroxy-3-methoxymandelic acid, 2-hydroxy-2-(4-hydroxy-3-methoxyphenyl)acetic acid, 4-HYDROXY-3-METHOXYMANDELIC ACID
CAS Number 55-10-7
PubChem ID 1245
Research Areas Biochemicals
Background Vanillylmandelic acid is an aromatic ether that is the 3-O-methyl ether of 3,4-dihydroxymandelic acid. It has a role as a human metabolite. It is an aromatic ether, a 2-hydroxy monocarboxylic acid and a member of phenols. It is functionally related to a mandelic acid. It is a conjugate acid of a vanillylmandelate.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.