VAMP 1,2/Synaptobrevin Monoclonal Antibody
Catalog No.: 56473 (clone SP-11)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
VAMP-1 (Vesicle-Associated Membrane Protein) is an 18kDa member of the synaptobrevin family. It is expressed in neurons, neutrophils, and skeletal muscle cells and participates in vesicle fusion with the plasma membrane. Synaptobrevins, syntaxins, SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP-1 is also one of the key proteins in the SNARE complex involved in regulated exocytosis.
Antigen: Crude human synaptic immunoprecipitate.
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human VAMP1 and VAMP2.
Marshak S et al. 2012. PLoS One 7: e33153.
Immunoblotting: use at 0.5-1.0ug/ml. A band of ~18kDa is detected.
Immunohistochemistry: use at 1-5ug/ml.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Human brain lysate
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in diagnostic or therapeutic procedures.