Anti-Ubiquitin Monoclonal Antibody
Catalog No.: 11023 (clone 5B9.B3)
Format: 100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Ubiquitin, a small protein comprised of 76 amino acids with a molecular mass of approximately 10kDa, is highly conserved among eukaryotic species. The primary role of ubiquitin is to clear abnormal, foreign, and improperly folded proteins by targeting them for degradation by the 26S proteosome. Ubiquitination of proteins is an essential process that is regulated by ubiquitin- activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. Ubiquitination also occurs in the internalization and degradation of plasma membrane proteins.
Antigen: Native bovine ubiquitin conjugated to KLH
Host Species: Mouse
Antibody Class: IgG2a
Accession no.: NP_776558.1
Gene ID: 281370
This antibody recognizes human, mouse, rat, and bovine ubiquitin. Other species have not been investigated.
Immunoblotting: use at 1ug/ml.
Immunofluorescence: use at 10ug/ml. A band of ~10kDa is detected.
ELISA: use at 1ug/ml with ubiquitin on the solid phase.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.