Anti-Tumor Necrosis Factorα Antibody (21303)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 200ug | ELISA,IHC | Human |  |
Overview
Product Name Anti-Tumor Necrosis Factorα Antibody (21303)
Description Anti-Tumor Necrosis Factorα Mouse Monoclonal Antibody
Target Tumor Necrosis Factorα
Species Reactivity Human
Applications ELISA,IHC
Host Mouse
Clonality Monoclonal
Clone ID F6C5
Isotype IgG1
Immunogen Recombinant human TNFa.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS containing 0.1% sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.1% Sodium Azide
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity 21303 recognizes a different epitope on TNFalpha than 21301 and 21302.21301 and 21302 can inhibit the biological activity of recombinant and native TNFalpha.
Target Name Tumor necrosis factor
Target ID Tumor Necrosis Factorα
Uniprot ID P01375
Alternative Names Cachectin, TNF-α, Tumor necrosis factor ligand superfamily member 2, TNF-a [Cleaved into: Tumor necrosis factor, membrane form
Gene Name TNF
Sequence Location Cell membrane, Single-pass type II membrane protein
Biological Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819). {UniProtKB:P06804, PubMed:12794819, PubMed:16829952, PubMed:22517918, PubMed:23396208}.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {PubMed:16829952}.
Research Areas Growth Factors, Cytokines, Receptors
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies may be used in immunoassays or in immunohisto- chemistry to detect and/or quantitate TNFa. 21301 and 21302 may be used in biological assays to inhibit activity of TNFa. Other applications are under investigation.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet