Anti-TSG101 Antibody (3352)
$503.00
Host | Quantity | Applications | Species Reactivity | Data Sheet | |
---|---|---|---|---|---|
Mouse | 100ul | WB,ICC/IF,IHC-P,FACS,IP,ELISA,EM,IHC,IHC | Human, Mouse, Rat, Hamster, Monkey |
SKU: 3352
Categories: Antibody Products, Cancer Research Antibodies, Products
Overview
Product Name Anti-TSG101 Antibody (3352)
Description Anti-TSG101 Mouse Monoclonal Antibody
Target TSG101
Species Reactivity Human, Mouse, Rat, Hamster, Monkey
Applications WB,ICC/IF,IHC-P,FACS,IP,ELISA,EM,IHC,IHC
Host Mouse
Clonality Monoclonal
Clone ID 4A10
Isotype IgG1
Immunogen Recombinant protein corresponding to aa 167-374 of TSG101 protein
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.2.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Format Purified
Purification Purified by affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, rat, hamster, and monkey TSG101 protein.
Target Name Tumor susceptibility gene 101 protein
Target ID TSG101
Uniprot ID Q99816
Alternative Names ESCRT-I complex subunit TSG101
Gene Name TSG101
Gene ID 7251
Accession Number NP_006283
Sequence Location Cytoplasm, Early endosome membrane, Late endosome membrane, Midbody, Midbody ring, Nucleus
Biological Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426). {PubMed:11916981, PubMed:17556548, PubMed:17853893, PubMed:21070952, PubMed:21757351, PubMed:22315426, PubMed:22660413}.
Research Areas Cancer Research
Application Images
Description TSG101 antibody [4A10] detects TSG101 protein by western blot analysis.
A. 30 ug NIH-3T3 whole cell lysate/extract
B. 30 ug JC whole cell lysate/extract
C. 30 ug BCL-1 whole cell lysate/extract
10% SDS-PAGE
TSG101 antibody [4A10] (3352) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
A. 30 ug NIH-3T3 whole cell lysate/extract
B. 30 ug JC whole cell lysate/extract
C. 30 ug BCL-1 whole cell lysate/extract
10% SDS-PAGE
TSG101 antibody [4A10] (3352) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description ELISA detection of TSG101 using for capture at a concentration of 5 ug/mL and 3352 for detection at a concentration of 1.5 ug/mL.
Description Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Description TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Description TSG101 antibody [4A10] (3352) detects TSG101 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: TSG101 antibody [4A10] (3352) dilution: 1:25.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: TSG101 antibody [4A10] (3352) dilution: 1:25.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
Description Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.
Description Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Description Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Handling
Storage This product is stable for at least one (1) year if stored at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions ELISA: use at 1-5ug/ml.
Immunoblotting: use at a 1:500-1:3,000 dilution. A band of 46kDa is detected. Detection of TSG101 protein in (A) NIH-3T3 cell lysate, (B) JC cell lysate, and (C) BCL-1 cell lysate with #3352 diluted 1:500.
Endusers should determine optimal antibody concentrations for their applications.
IHC: use at a dilution of 1:100 with antigen retrieval: citrate buffer, pH 6.0, 15 minutes. Detection of TSG101 protein in paraffin-embedded human breast carcinoma. Detection of TSG101 protein in paraffin-embedded human ovarian carcinoma.
Immunoblotting: use at a 1:500-1:3,000 dilution. A band of 46kDa is detected. Detection of TSG101 protein in (A) NIH-3T3 cell lysate, (B) JC cell lysate, and (C) BCL-1 cell lysate with #3352 diluted 1:500.
Endusers should determine optimal antibody concentrations for their applications.
IHC: use at a dilution of 1:100 with antigen retrieval: citrate buffer, pH 6.0, 15 minutes. Detection of TSG101 protein in paraffin-embedded human breast carcinoma. Detection of TSG101 protein in paraffin-embedded human ovarian carcinoma.
References & Data Sheet
Data Sheet Download PDF Data Sheet