Anti-TrpV3 Cation Channel Monoclonal Antibody
Catalog No.: 11527 (clone S15-39)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. TrpV3 is in a family of non- selective cation channels that function in a variety of processes including temperature sensation and vaso-regulation. The thermosensitive members of this family are expressed in sensory neurons that terminate in the skin and is activated at temperatures between 22-40oC. TrpV3 might associate with TrpV1 to form heteromeric channels.
Antigen: Synthetic peptide corresponding to aa 458-474 (cytoplasmic C-terminus) of rat TrpV3 (accession no. NP_001020928). This sequence is 77% homologous with human and 100% homologous with mouse TrpV3.
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human, mouse, and rat TrpV3.
Immunoblotting: use at 1ug/ml. A band of ~70kDa is detected.
Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml
Immunofluorescence: use at 1-10ug/ml
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Lysate of COS-1 cells transiently-transfected with TrpV3.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.