Anti-TrpM7 Ion Channel and Ser/Thr Kinase Monoclonal Antibody
Catalog No.: 11519 (clone S74-25)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. TRPs, mammalian homologs of the Drosophilia transient receptor potential (trp) proteins, are ion channels that are thought to mediate capacitative calcium entry into cells. TrpM7 functions as an ion channel and a serine/threonine kinase. As an ion channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it can phosphorylate itself and other substrates. This kinase activity is necessary for channel function.
Antigen: Fusion protein aa 1817-1863 (cytoplasmic C-terminus) of mouse TrpM7 (accession no. Q923J1).
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human, mouse, and rat TrpM7. It does not cross-react with TrpM6.
Immunoblotting: use at 1-10ug/ml. A band of ~220kDa is detected.
Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml
Immunofluorescence: use at 1-10ug/ml
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: COS cell lysate transiently transfected with TrpM7.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.