recomLine Tropical Fever IgM
The first immunoblot assay for simultaneous detection and differentiation of Dengue, Chikungunya and Zika antibodies. CE marked, Patent Pending
recomLine Tropical Fever Recombinant Antigens
As the Zika virus epidemic continues to spread, reliable differentiation of Zika virus from related viruses has been challenging, particularly in areas with a long history of endemic Dengue. Both Zika virus and Dengue virus belong to the same family of flaviviruses. Many Dengue assays detect antibodies to the viral envelope (E) protein which cross-react with E protein of other flaviviruses such as Zika (ZIKV), Yellow Fever (YFV), West Nile Fever (WNV), Tick-borne Encephalitis (TBE), and Japanese Encephalitis (JEV). However, detection of antibody to the NS1 protein has been reported to have minimal cross-reactivity with NS1 proteins of different flaviviruses. The recomLine Tropical Fever immunoblot combines NS1 and E proteins into one assay in order to distinguish antibodies to Dengue, Chikungunya and Zika viral proteins.
#7872 (#7870) recomLine Tropical Fever IgG Reagents for 20 (100) determinations
#7873 (#7879) recomLine Tropical Fever IgM Reagents for 20 (100) determinations
Available in the U.S. For Research Use Only. Not intended for diagnostic procedures.
Test Principle and Procedure
1st Incubation A test strip loaded with DENV, CHIKV and ZIKV antigens is incubated with diluted serum or plasma in a dish or tube for 1 hour.
2nd Incubation Peroxidase conjugated anti-human antibodies (IgG or IgM specific) are added. Incubate for 45 minutes.
Color reaction 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips where antibodies are bound.