Anti-TRAP1/Hsp75 Antibody (11136)

$448.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF Human
SKU: 11136 Categories: , ,
Overview
Product Name Anti-TRAP1/Hsp75 Antibody (11136)
Description Anti-TRAP1 Mouse Monoclonal Antibody
Target TRAP1/Hsp75
Species Reactivity Human
Applications WB,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID Trap1-6
Isotype IgG2a
Immunogen Recombinant human TRAP1
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human TRAP1. Other species have not been investigated.
Target Name Heat shock protein 75 kDa, mitochondrial
Target ID TRAP1/Hsp75
Uniprot ID Q12931
Alternative Names HSP 75, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1
Gene Name TRAP1
Gene ID 10131
Accession Number NP_057376.2
Sequence Location Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix
Biological Function Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. {PubMed:23525905, PubMed:23564345, PubMed:23747254}.
Research Areas Heat Shock& Stress Proteins
Background The molecular chaperone family of 90kDa heat shock proteins (Hsp90) is a highly conserved protein family that plays an important physiological role. Hsp90 is best known for its association with signal transduction processes. Tumor necrosis factor receptor-associated protein 1 (TRAP1) is a recently cloned homolog of Hsp90. Like Hsp90, TRAP1 is associated with numerous proteins that are involved in multiple pathways. Data indicates that TRAP1 is a mitochondrial matrix protein: it is localized in mitochondria of mammalian cells and contains a mitochondrial targeting presequence.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6 (11136). Tissue: PC3M cells. Species: Human. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (11136) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Mitotracker (red) mitochondria stain. DAPI (blue) nuclear stain. (A) staining of mitochondria with MitoTracker dye; (B) staining of nuclei with DAPI; (C) staining with TRAP1 antibody; (D) tricolor composite of images A-C.
Description Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6 (11136). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (11136) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~75kDa is detected.

Immunocytochemistry: use at 0.1-1.0ug/mL.

Immunofluorescence: use at 1-10ug/mL.

Positive control: HeLa cells or lysate

These are recommended concentrations;

Enduser should determine optimal concentrations for their applications.
References & Data Sheet