Transglutaminase-3 (TG3) Monoclonal Antibody
Catalog No.: 90022 (clone C2D)
Format: 100ug, Protein A-purified, lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Transglutaminase-3 (TG3), an epidermal- type enzyme, is responsible for formation of the epidermis. During keratinocyte differentiation, TG1 and TG3 appear to act cooperatively in the cross-linking of proteins, including involucrin, loricrin and small proline-rich proteins (SPRs). These reactions result in formation of the cornified envelope (CE), a specialized component consisting of covalent cross- links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Furthermore, TG3 in hair follicles is involved in cross-linking structural proteins such as trichohyalin and keratin intermediate to hardening the inner root sheath. During differentiation in these processes, a zymogen form of TG3 (77 kDa) is activated by limited proteolysis with cathepsins S and/or L.
Antigen: Recombinant human TG3 (accession no. Q08188).
Host species: Mouse.
Antibody Class: IgG1.
This antibody recognizes full-length human TG3 (77kDa) and the proteolysed form of human TG3 (30-47kDa).
Immunoblotting: use at 1-5ug/ml. Bands of ~77kDa and ~30kDa are detected.
Immunocytochemistry: use at 2-10ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Reconstitute in distilled water.
STORAGE AND STABILITY
This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.