Anti-Transcription Factor IIB Antibody (70103)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 200ug | WB,ELISA,Gel Shift,Inhibition | Human, Mouse |  |
Overview
Product Name Anti-Transcription Factor IIB Antibody (70103)
Description Anti-Transcription Factor IIB Mouse Monoclonal Antibody
Target Transcription Factor IIB
Species Reactivity Human, Mouse
Applications WB,ELISA,Gel Shift,Inhibition
Host Mouse
Clonality Monoclonal
Clone ID IIB8
Isotype IgG2a
Immunogen Partially purified recombinant human transcription factor IIB (TFIIB, Promega Corp.).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes an epitope within amino acids 52-105 of human and mouse TFIIB.
Target Name Transcription initiation factor IIB
Target ID Transcription Factor IIB
Uniprot ID Q00403
Alternative Names EC 2.3.1.48, General transcription factor TFIIB, S300-II
Gene Name GTF2B
Sequence Location Nucleus, Chromosome
Biological Function General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-rPubMed:10318856, PubMed:10619841, PubMed:12931194, PubMed:1517211, PubMed:16230532, PubMed:1876184, PubMed:1946368, PubMed:24441171, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:7675079, PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329}.
Research Areas Protein Transcription
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70 C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions This antibody may be used in ELISA, Western blot, or electrophoretic mobility (gel shift) assays to identify human TFIIB. It can also immuno-deplet TFIIB from cell nuclear extracts and inhibit transcription of TFIIB from cell nuclear extracts. This antibody is polyol-responsive and can be used for gentle purification of TFIIB.
References & Data Sheet
References Thompson NE and Burgess RR. 1994. Protein Expression Purification 5: 468- 475
Thompson NE et al. 1995. J Biol Chem 270: 4735-4740.
Thompson NE et al. 1995. J Biol Chem 270: 4735-4740.
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet