Toll-like Receptor 3 (TLR3) Polyclonal Antibody
Catalog No.: 56503
Size: 50 ug purified by antigen affinity chromatography in PBS containing 0.02% sodium azide.
TLR3 is a 904 aa protein with 24 N-terminal leucine-rich repeat motifs (LRRs) and a molecular weight of 97 kDa. TLR3 is most closely related to TLR5, TLR7, and TLR8, each with 26% overall sequence identity. In vivo, two different sized transcripts for TLR3 are observed suggesting that the mRNA is alternatively spliced to generate two different forms of the protein. TLR3 mRNA is expressed at highest levels in placenta and pancreas. There are conflicting reports regarding the expression of TLR3 in particular leukocyte populations. Some suggest that TLR3 is only expressed by dendritic cells (DCs), while others find that TLR3 is expressed by T or NK cells. While it is generally assumed that TLRs are expressed on the cell surface, it now appears that some, such as TLR3, may be localized intracellularly, perhaps in the lysosomal compartment.
Antigen: Synthetic peptide corresponding to 15 amino acids near the N-terminus of human TLR3.
Host Species: Rabbit
Antibody Class: Polyclonal
Preservatives: 0.02% sodium azide
This antibody recognizes human and mouse TLR3.
Western blotting: use at 1-2ug/ml. A band of approximately 97 kDa is detected.
Immunohistochemistry: formalin-fixed, paraffin-embedded tissues, use at 5ug/ml.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
Store at 4°C. Avoid freezing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.