Thyroxine ELISA Kit (ECP7948)
$479.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name Thyroxine ELISA Kit (ECP7948)
Description Thyroxine ELISA Kit (ECP7948) is a Competitive Inhibition ELISA for the quantitiation of Thyroxine in a variety of aqueous sample types.
Target Thyroxine
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 776.87 g·mol−1
Background Minimum Level of Detection: 1.29 ng/mL
Dynamic range of quantitation: 3.70-300 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 3.70-300 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Thyroxine Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Thyroxine Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 Vial |
| Resuspension Solution A | 1 x 300uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| Stop Solution | 1 x 6mL |
| TMB Substrate | 1 x 9mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Thyroxine
Alternative Names L-thyroxine, levothyroxine, 51-48-9, thyroxine, thyroxin
CAS Number 51-48-9
PubChem ID 5819
Research Areas Biochemicals
Background Levothyroxine is a synthetic levoisomer of thyroxine (T4), similar to the endogenous hormone produced by the thyroid gland. Thyroxine is de-iodinated to form triiodothyronine (T3) in the peripheral tissues. T3 enters the cell and binds to nuclear thyroid hormone receptors, and the hormone-receptor complex in turn triggers gene expression and produces proteins required in the regulation of cellular respiration, thermogenesis, cellular growth and differentiation, and metabolism of proteins, carbohydrates and lipids. T4 and T3 also possess cardiac stimulatory effect.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.