Tetrahydrobiopterin ELISA Kit (ECP7955)
$792.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name Tetrahydrobiopterin ELISA Kit (ECP7955)
Description Tetrahydrobiopterin ELISA Kit (ECP7955) is a Competitive Inhibition ELISA for the quantitiation of Tetrahydrobiopterin in a variety of aqueous sample types.
Target Tetrahydrobiopterin
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 241.251 g·mol−1
Background Minimum Level of Detection: 155 pg/mL
Dynamic range of quantitation: 391-100,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 391-100,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Tetrahydrobiopterin Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Tetrahydrobiopterin Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 x 120uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| TMB Substrate | 1 x 9mL |
| Stop Solution | 1 x 6mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Tetrahydrobiopterin
Alternative Names 5,6,7,8-tetrahydrobiopterin, 2-amino-6-(1,2-dihydroxypropyl)-5,6,7,8-tetrahydropteridin-4(3H)-one, CHEBI:15372
CAS Number 62989-33-7
PubChem ID 135402045
Research Areas Biochemicals
Background 5,6,7,8-tetrahydrobiopterin is a tetrahydropterin, a member of biopterins and a diol. It has a role as a prosthetic group, a coenzyme, a nutraceutical and a human metabolite.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.