Anti-Tau Antibody

$594.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,DB,ICC/IF,ELISA Human, Mouse Rat
SKU: 57012 Categories: , ,
Overview
Product Name Anti-Tau Antibody (57012)
Description Anti-Tau Mouse Monoclonal Antibody
Target Tau
Species Reactivity Human, Mouse Rat
Applications WB,DB,ICC/IF,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 1D5
Immunogen Full-length recombinant human Tau441 (2N4R) P301S mutant protein pre-formed fibrils. In humans, mutation P301S gives rise to early-onset dementia.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Tau.
Target Name Microtubule-associated protein tau
Target ID Tau
Uniprot ID P10636
Alternative Names Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Gene Name MAPT
Sequence Location Cytoplasm, cytosol, Cell membrane, Cytoplasm, cytoskeleton, Cell projection, axon, Cell projection, dendrite, Secreted
Biological Function Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. {PubMed:21985311, PubMed:32961270}.
Application Images
Description Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (57012). Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (57012) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.
Description munocytochemistry/Immunofluorescence analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (57012). Tissue: iPSC-derived neurons. Species: Human. Fixation: 4% PFA. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (57012) at 1:100 for Overnight at 4°C. Counterstain: DAPI at 1:5000 for 5 minutes at RT in the dark. Magnification: 40X. Courtesy of: Francesco Paonessa.
Description Western Blot analysis of Human Breast Cancer Cell line and Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (57012). Lane 1: MW Marker. Lane 2: Human T-47d (10ug). Lane 3: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (57012) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.
Additional Information
Handling
Storage Store at 2 - 8°C. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5ug/mL. A band of ~45.8kDa is detected.
References & Data Sheet