Synaptotagmin-6 Monoclonal Antibody
Catalog No.: 56532 (clone S270-47)
Size: 100ug in PBS (1mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N terminal trans- membrane region (TMR), a variable linker,and two C-terminal C2 domains: C2A and C2B. There are 15 members in the mammalian synaptotagmin family. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to act as Ca+2 sensors in vesicular trafficking and exocytosis. Calcium binding to synaptotagmin triggers neurotransmitter release at the synapse. The first C2 domain mediates Ca+2-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synapto- tagmin-6 is localized to the endoplasmic reticulum and/or Golgi-like perinuclear compartment. It is also present in the acrosomal region of mammalian spermatozoa.
Antigen: Fusion protein corresponding to aa 246-333 of mouse Synapto- tagmin-6 (accession no.Q9R0N8).
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human, mouse and rat Synaptotagmin-6. It does not cross-react with other Synaptotagmins.
Immunoblotting: use at 1ug/ml. Predicted molecular weight is ~55kDa.
Positive Control: rat brain lysate.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze- thaw cycles.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.