Anti-Superoxide dismutase 1 Antibody (13065)

$448.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF,ELISA Human, Mouse, Rat
SKU: 13065 Categories: , ,
Overview
Product Name Anti-Superoxide dismutase 1 Antibody (13065)
Description Anti-Superoxide dismutase 1 (SOD1) Rabbit Polyclonal Antibody
Target Superoxide dismutase 1
Species Reactivity Human, Mouse, Rat
Applications WB,ICC/IF,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Peptide corresponding to unfolded beta barrel (UβB) region at the N-terminus of SOD1
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by protein A affinity chromatography
Specificity Information
Target Name Superoxide dismutase [Cu-Zn]
Target ID Superoxide dismutase 1
Uniprot ID P00441
Alternative Names EC 1.15.1.1, Superoxide dismutase 1, hSod1
Gene Name SOD1
Accession Number NP_000445
Sequence Location Cytoplasm, Mitochondrion, Nucleus
Biological Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems. {PubMed:24140062}.
Research Areas Enzymes
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (13065). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (13065) at 1:110 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD1 (UBB) Antibody. (C) Composite.
Description Western blot analysis of Human, Rat, Mouse Hela cells, Brain, Lung showing detection of ~18 kDa SOD1 (UBB) protein using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (13065). Lane 1: Molecular Weight Ladder. Lane 2: Human HeLa Cell Lysates. Lane 3: Rat Brain. Lane 4: Mouse Lung. Load: 20 µg. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (13065) at 1:1000. Predicted/Observed Size: ~18 kDa.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~18kDa is detected.

Immunofluorescence: use at 10ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet