Sphingosine ELISA Kit (ECP7945)
$722.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name Sphingosine ELISA Kit (ECP7945)
Description Sphingosine ELISA Kit (ECP7945) is a Competitive Inhibition ELISA for the quantitiation of Sphingosine in a variety of aqueous sample types.
Target Sphingosine
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 299.499 g·mol−1
Background Minimum Level of Detection: 2.67 ng/mL
Dynamic range of quantitation: 6.17-500 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 6.17-500 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Sphingosine Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Sphingosine Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 x 120uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| TMB Substrate | 1 x 9mL |
| Stop Solution | 1 x 6mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Sphingosine
Alternative Names (2S,3R,4E)-2-Aminooctadec-4-ene-1,3-diol
CAS Number 123-78-4
PubChem ID 5280335
Research Areas Biochemicals
Background Sphingosine is a sphing-4-enine in which the double bond is trans. It has a role as a human metabolite and a mouse metabolite. It is a conjugate base of a sphingosine(1+). It is an enantiomer of a L-erythro-sphingosine.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.