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Anti-SOX6 Antibody (28117)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB Human, Mouse, Rat, Monkey
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SKU: 28117 Categories: , ,
Overview
Product Name Anti-SOX6 Antibody (28117)
Description Anti-SOX6 Rabbit Polyclonal Antibody
Target SOX6
Species Reactivity Human, Mouse, Rat, Monkey
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids 780-796 of human and mouse SOX6.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human SOX6 and is predicted to recognize mouse, rat, and chimpanzee SOX6 based on 100% sequence homology.
Target Name Transcription factor SOX-6
Target ID SOX6
Uniprot ID P35712
Gene Name SOX6
Sequence Location Nucleus, Cytoplasm
Biological Function Transcription factor that plays a key role in several developmental processes, including neurogenesis, chondrocytes differentiation and cartilage formation (Probable). Specifically binds the 5'-AACAAT-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis. Required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes: SOX5 and SOX6 cooperatively bind with SOX9 on active enhancers and super-enhancers associated with cartilage-specific genes, and thereby potentiate SOX9's ability to transactivate. Not involved in precartilaginous condensation, the first step in chondrogenesis, during which skeletal progenitors differentiate into prechondrocytes. Together with SOX5, required to form and maintain a pool of highly proliferating chondroblasts between epiphyses and metaphyses, to form columnar chondroblasts, delay chondrocyte prehypertrophy but promote hypertrophy, and to delay terminal differentiation of chondrocytes on contact with ossification fronts. Binds to the proximal promoter region of the myelin protein MPZ gene, and is thereby involved in the differentiation of oligodendroglia in the developing spinal tube. Binds to the gene promoter of MBP and acts as a transcriptional repressor (By similarity). {UniProtKB:P40645, PubMed:32442410}.
Research Areas Neuroscience
Background The SOX gene family is defined by the Sry-related HMG box domain that mediates sequence-specific DNA binding. A variety of functions are associated with the SOX proteins during development. SOX6 is believed to regulate embryonic development and determine cell fate. Mouse SOX6 is expressed in the CNS during embryogenesis, but this expression decreases significantly in the adult CNS. In humans, SOX6 is expressed in fetal brain and in gliomas but only at very low levels in the adult brain. A direct link between SOX6 expression and malignancy has not been demonstrated, so SOX6 probably does not promote proliferation, but it may induce transformation activity in the early stage of oncogenesis in gliomas.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1-5ug/ml. A band of approximately 93 kDa is detected.

These are recommended concentrations. User should determine optimal concentrations for their applications.
References & Data Sheet
References Beilina, A et al. (2005) Proc Natl Acad Sci USA 102: 5703-08.Valente, EM et al. (2004) Science 304: 1158-60.Rogaeva, E et al. (2004) Arch Neurol 61: 1898-1904.Hatano, Y et al. (2004) Ann Neurol 56: 424-27.