SOX2 Monoclonal Antibody
Catalog no.: 11301
Format: 100ug lyophilized from purified antibody in 10mM PBS, pH 7.4, 1.0% bovine serum albumin (BSA).
The SOX2 gene codes for SOX2 protein (also called SRY-box2) that plays a critical role in the formation of many different tissues and organs during embryonic development. The SOX2 protein is especially important for the development of the eyes. This protein family shares highly conserved DNA binding domains known as HMG (High-mobility group) box domains containing approximately 80 amino acids.
Antigen: Synthetic peptide corresponding to aa 219-236 (GSPTYSMSYSQQGTPGMA) of human SOX2.
Accession no.: P48431
Gene ID: 6657
Antibody Class: IgG2b
Clone no.: 9B12H
Host Species: Mouse
Specificity: This antibody recognizes human SOX2.
Immunoblotting: use at 1-5ug/ml under reducing conditions. A band of ~34kDa is detected.
Immunofluorescence: use at 0.2-1ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dissolve the lyophilized antibody in 50ul of distilled water (final concentration 2.0mg/ml) for stock solution. Dilute the stock solution in 10mM PBS, pH 7.4 + 1.0% BSA immediately before use.
STORAGE AND STABILITY
Store the stock solution at a minimum concentration of 2.0mg/ml. A lower concentration may result in decreased stability. Store stock solution at -20ºC for at least one (1) year; avoid multiple freeze-thaw cycles. Add 0.1% sodium azide in order to store stock solution at 4ºC. Diluted antibody should not be stored.
For in vitro investigational use only. Not intended for therapeutic or diagnostic procedures.