Anti-SOX-10 Antibody (34071)

$248.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ul IHC Human
SKU: 34071 Categories: , , Tag:
Overview
Product Name Anti-SOX-10 Antibody (34071)
Description Anti-SOX-10 Mouse Monoclonal Antibody
Target SOX-10
Species Reactivity Human
Applications IHC
Host Mouse
Clonality Monoclonal
Clone ID G010.1
Isotype IgG2b
Immunogen Recombinant human SOX-10.
Properties
Form Liquid
Concentration Lot Specific
Formulation Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
Buffer Formulation Tris
Buffer pH pH 7.3-7.7
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity Human SOX-10. Reactivity with other species has not been investigated.
Target Name Transcription factor SOX-10
Target ID SOX-10
Uniprot ID P56693
Gene Name SOX10
Sequence Location Cytoplasm, Nucleus, Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side
Biological Function Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity). {UniProtKB:O55170, UniProtKB:Q04888, PubMed:21965087}.
Research Areas Cancer research
Background SRY (Sex Determining Region Y)-Box 10 (SOX-10), also known as transcription factor SOX-10, is a nuclear transcription factor that acts in regulation of embryonic development and in the specification and differentiation of cells of melanocytic lineage. SOX-10 is diffusely expressed in neurobromas and schwannomas, and mutations in the SOX-10 gene are linked to Waardenburg- Shah and Waardenburg-Hirschsprung disease.
Application Images
Description Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes. Detection of SOX-10 in human melanoma with #34071 diluted 1:100-1:200.
Additional Information
Handling
Storage Store at 2-8°C. Do not freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
References & Data Sheet