Anti-SNAP25 Monoclonal Antibody
Catalog No.: 56480 (clone SP-12)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Synaptosomal-associated protein 25 (SNAP25) is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. It is a component of the SNARE complex which is believed to account for the specificity of membrane fusion and for executing fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. Two alternatively spliced isoforms of SNAP25 have been identified; SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and differ by 9 amino acids in the central portion of the protein.
Antigen: Crude synaptic preparation from post-mortem human brain.
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes human, mouse, rat, feline, and porcine SNAP25.
Immunoblotting: use at 1-10ug/ml. A band of ~29kDa is detected.
ELISA: use at 1-5ug/ml
Immunohistochemistry: use at 1-10ug/ml
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.