Anti-ski Monoclonal Antibody
Catalog No.: 34021 (clone G8) Size: 100 ug Protein G-purified antibody in PBS, pH 7.4
The ski oncogene family (ski and sno) encodes transcriptional regulators that can affect oncogenic transformation and cellular differenti- ation. ski and sno function as co- activators or co-repressors, modulating transcription by inter- acting with transcription factor complexes. ski interacts with members of the Nuclear Factor I (NFI) family, the retinoic acid receptor, and the nuclear hormone co-regulators NCoR and NCoA62/skip.
Immunogen: Recombinant v-ski
Host Species: Mouse
Antibody Class: IgG1
Preservatives: None. Available on request.
This antibody recognizes ski protein of human, mouse, rat, chicken, and Xenopus.
Immunoblotting: use at 1-5 ug/ml. Predicted molecular weight of v-ski is 55 kDa; full-length ski ranges from ~95 kDa up to ~115 kDa for hyperphosphorylated ski.
Immunocytochemistry: use at 1-10 ug/ml. ski is a nuclear protein.
Immunoprecipitation: use at 1-5 ug/ml. Positive controls: Recombinant cell lines expressing ski proteins.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1)year at -70°C. Avoid multiple freeze- thaw cycles.
Tarapore, P et al. (1997) Nucleic Acids Res 25: 3895-3903.
Colmenares, C et al. (1991) Molecular and Cellular Biology 11: 1167-70.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.