Anti-SIRPα Antibody (2428)

$464.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC Human, Mouse, Rat, Bovine
SKU: 2428 Categories: , ,
Overview
Product Name Anti-SIRPα Antibody (2428)
Description Anti-SIRPα (CT) Rabbit Polyclonal Antibody
Target SIRPα
Species Reactivity Human, Mouse, Rat, Bovine
Applications ELISA,WB,ICC
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic peptide corresponding to 17 amino acids within the last 50 amino acids at the C-terminus of human SIRPa.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, 0.02% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.02% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human SIRP alpha 1, 2, and 3.
Target Name Tyrosine-protein phosphatase non-receptor type substrate 1
Target ID SIRPα
Uniprot ID P78324
Alternative Names SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory proteinα-1, Sirp-α-1, Signal-regulatory proteinα-2, Sirp-α-2, Signal-regulatory proteinα-3, Sirp-α-3, p84, CD antigen CD172a
Gene Name SIRPA
Gene ID 140885
Accession Number NP_542970
Sequence Location Membrane; Single-pass type I membrane protein.
Biological Function Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. {PubMed:10469599, PubMed:11509594}.
Research Areas Neuroscience
Background Protein tyrosine phosphatases (PTPases), such as SHP-1 and SHP-2, are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation, or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was cloned from human, rat, mouse, and cattle by several laboratories and was designated SIRPalpha, SHPS-1, and MyD-1. SIRPalpha/SHPS-1 is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR, and src, and is a specific docking protein for SHP-2. SIRPalpha/SHPS-1 has regulatory effects on cellular responses and plays a general role in different physiological and pathological processes.
Application Images
Description Western blot analysis of SIRP alpha in THP-1 whole cell lysate with SIRP alpha antibody at 0.5 ug/mL.
Description Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 ug/mL.
Additional Information
Handling
Storage This antibody is stable at 4°C for three months and for at least one year at -20ËšC. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-2ug/mL. A band of 75-110kDa is detected.

Immunocytochemistry: use at 1-5ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet