SARS-CoV-2 IgA recomWell

 1 Purpose 

For sale for Research Use Only. Not for diagnostic use. 

2 Intended Use 

MIKROGEN’s recomWell SARS-Cov-2 IgA detects IgA antibodies against SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). This test must not be used for clinical diagnosis! 

3 Test Principle 

Highly purified recombinant nucleocapsid protein of SARS-CoV-2 is fixed to the wells of a microplate. 

1. Diluted serum or plasma samples are incubated in the wells; antibodies in the samples bind specifically to the antigen on the wells. 

2. Unbound antibodies are washed away. 

3. In a second step, anti-Human Immunoglobulin antibody, coupled to horseradish peroxidase (HRP) is incubated in the wells. 

4. Unbound conjugate is washed away. 

5. Specifically-bound antibodies are detected by a peroxidase-catalyzed color reaction. Where an antigen-antibody reaction has taken place, the color development of the chromogenic substrate is proportional to the quantity of bound SARS-CoV-2 IgA antibodies. The concentration of color development can be measured using a photometer so as to establish the concentration of anti- SARS-CoV-2 IgA antibodies in the samples.