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Anti-SAP30L Antibody (28113)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ELISA Human
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Overview
Product Name Anti-SAP30L Antibody (28113)
Description Anti-SAP30L Rabbit Polyclonal Antibody
Target SAP30L
Species Reactivity Human
Applications WB,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids 165-183 (VKSNKSRLDQKSEGGKQLE) of human SAP30L conjugated to KLH at the N- terminus.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody detects wild-type transfected, endogenous, or GST- SAP30L fusion protein in human cell lines.
Target Name Histone deacetylase complex subunit SAP30L
Target ID SAP30L
Uniprot ID Q9HAJ7
Alternative Names HCV non-structural protein 4A-transactivated protein 2, Sin3 corepressor complex subunit SAP30L, Sin3-associated protein p30-like
Gene Name SAP30L
Sequence Location [Isoform 1]: Nucleus, nucleolus
Biological Function [Isoform 1]: Functions as transcription repressor, probably via its interaction with histone deacetylase complexes (PubMed:16820529, PubMed:18070604). Involved in the functional recruitment of the class 1 Sin3-histone deacetylase complex (HDAC) to the nucleolus (PubMed:16820529). Binds DNA, apparently without sequence-specificity, and bends bound double-stranded DNA (PubMed:19015240). Binds phosphoinositol phosphates (phosphoinositol 3-phosphate, phosphoinositol 4-phosphate and phosphoinositol 5-phosphate) via the same basic sequence motif that mediates DNA binding and nuclear import (PubMed:19015240, PubMed:26609676). {PubMed:16820529, PubMed:18070604, PubMed:19015240, PubMed:26609676}.; [Isoform 2]: Functions as transcription repressor; isoform 2 has lower transcription repressor activity than isoform 1 and isoform 3. {PubMed:18070604}.; [Isoform 3]: Functions as transcription repressor; its activity is marginally lower than that of isoform 1. {PubMed:18070604}.
Research Areas Neuroscience
Background A novel TGF-beta up-regulated mRNA species, the Sin3-associated protein 30- like, SAP30L has been identified. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. By reason of its nuclear localization and close homology to SAP30, it is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co- repressor complexes in response to TGF- beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500 dilution. Bands of approximately 21-22 kDa (endogenous) and/or 30 kDa (wild-type transfected) are detected.

Positive control: MCF7 or HEK293 cell lysate.
References & Data Sheet
References Lindfors,K. et al. BMC Genomics 4 (1), 53 (2003).