S100A1 Polyclonal Antibody
Catalog No.: 56201
Size: 200 ul IgG prepared by ammonium sulfate precipitation in PBS, pH 7.4.
Antigen: Alum denatured porcine S100A1.
Host Species: Sheep
Preservatives: None. Available upon request.
This antibody reacts with bovine and porcine S100A1 in immunoblots. Immunoreactivity with recombinant rat and human S100A1 and recombinant human S100A4 is reduced when compared to native bovine and porcine S100A1. Does not react with human S100A2 in immunoblots. This antibody reacts with bovine, rat, and recombinant S100B in immunoblots but less than 5% cross- reactivity with S100B is seen in competitition radioimmunoassays. Cross- reactivity with other members of the S100 family has not been investigated.
This antibody may be used in immunohistochemistry, immuno- cytochemistry, radioimmunoassays, and Western blots to detect or quantitate S100A1.
Dilute in PBS or medium which is identical to that used in the assay system. For Western blots using an avidin-biotin secondary antibody system, a 1:50 dilution has been used successfully. For indirect immunofluorescence, a 1:2 dilution has been used successfully. Optimal dilutions should be determined for each assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Zimmer and Landar. 1995. Jour Neurochemistry 64: 2727;
Van Eldik et al. 1988. Jour Biol Chem 263: 7830.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.