Anti-S100α/β Antibody (56203)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA,AP Human
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SKU: 56203 Categories: , ,
Overview
Product Name Anti-S100α/β Antibody (56203)
Description Anti-S-100 Mouse Monoclonal Antibody
Target S100α/β
Species Reactivity Human
Applications WB,ELISA,AP
Host Mouse
Clonality Monoclonal
Clone ID 8B10
Isotype IgG1
Immunogen Purified S-100 protein from human brain.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4, 0.09% sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity 56203, 56204, and 56205 recognize S-100alphab and S-100 bb. 56206 recognizes S-100alphab.
Target Name Protein S100-A1
Target ID S100α/β
Uniprot ID P23297
Alternative Names S-100 proteinα chain, S-100 protein subunitα, S100 calcium-binding protein A1
Gene Name S100A1
Sequence Location Cytoplasm, Sarcoplasmic reticulum, Mitochondrion
Biological Function Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446). {PubMed:11717446, PubMed:12804600, PubMed:22399290, PubMed:23351007}.
Research Areas Neuroscience
Application Images
Description 20% PAGE w/ 2mM EGTA, 1 ug Antigen.
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions These antibodies may be used in ELISA and Western blot (human brain): 56203, 56204, and 56206 may be used to affinity-purify S-100.Recommended pairs for sandwich ELISA: Capture Detect 56203 56204 56206 56204
References & Data Sheet
References Zimmer and Landar. 1995. Jour Neurochemistry 64: 2727
Van Eldik et al. 1988. Jour Biol Chem 263: 7830.