RPA32 Monoclonal Antibody
Catalog No.: 56216
Size: 100 ug Protein G-purified antibody in PBS, pH 7.4
Antigen: N-terminally 6x-His tagged fusion protein corresponding to full-length human RPA32 expressed in E. coli.
Host Species: Mouse
Antibody Class: IgG2a/κ
Preservatives: None. Available on request.
This antibody recognizes human and mouse RPA32. RPA (Replication Protein A) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kD. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair. RPA32 is phosphorylated during the S-phase of the cell cycle, in response to DNA damage, and during apoptosis of Jurkat T-lymphocytes.
Immunoblotting, immunoprecipitation: use at 0.2-1 ug/ml.
Positive control: HeLa, Raji, and 3T3 cells and recombinant protein.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.
Iftode, C et al. 1999. Crit Rev Biochem Mol Biol 34: 141-180;
Bochkarev, A et al. 1999. EMBO 18: 4498-4504;
Dong, J et al. 1999. Biochem J 337: 311-317;
Sibenaller, ZA et al. 1998. Biochemistry 37: 12496-12506;
Stigger, E et al. 1998. J Biol Chem 273: 9337-9343;
Bochkareva, E et al. 1998. J Biol Chem 273: 3932-3936.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.