Anti-RPA14 Antibody (56215)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC-P,IP Human, Mouse
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Overview
Product Name Anti-RPA14 Antibody (56215)
Description Anti-RPA14 Mouse Monoclonal Antibody
Target RPA14
Species Reactivity Human, Mouse
Applications WB,IHC-P,IP
Host Mouse
Clonality Monoclonal
Clone ID 11.1
Isotype IgG1
Immunogen N-terminally 6x-His tagged fusion protein corresponding to full-length human RPA14 expressed in E. coli.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse RPA14. RPA (Replication Protein A) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kD. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair.
Target Name Replication protein A 14 kDa subunit
Target ID RPA14
Uniprot ID P35244
Alternative Names RP-A p14, Replication factor A protein 3, RF-A protein 3
Gene Name RPA3
Sequence Location Nucleus
Biological Function As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER), probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). {PubMed:19010961, PubMed:19116208, PubMed:19996105, PubMed:7697716, PubMed:9430682, PubMed:9765279, PubMed:24332808}.
Research Areas Cancer research
Application Images
Description RPA14 antibody [11.1] detects RPA14 protein at nucleus on Mahlarvu xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Mahlarvu xenograft.
RPA14 antibody [11.1] (56215) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Description RPA14 antibody [11.1] detects RPA14 protein by Western blot analysis.
A. 30 ug A549 whole cell lysate/extract
B. 30 ug MCF-7 whole cell lysate/extract
15 % SDS-PAGE
RPA14 antibody [11.1] (56215) dilution: 1:1000
Description Sample (30 ug of whole cell lysate)
A: MCF7
15% SDS PAGE
56215 diluted at 1:500
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting,

Immunoprecipitation: use at 0.2-1 ug/mL.

Positive control: HeLa, Raji, and 3T3 cells and recombinant protein.
References & Data Sheet
References Iftode, C et al. 1999. Crit Rev Biochem Mol Biol 34: 141-180
Bochkarev, A et al. 1999. EMBO 18: 4498-4504
Dong, J et al. 1999. Biochem J 337: 311-317
Sibenaller, ZA et al. 1998. Biochemistry 37: 12496-12506
Stigger, E et al. 1998. J Biol Chem 273: 9337-9343.