Anti-Rotavirus Group Antigen Antibody (20601)

$270.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA,IF Rotavirus Group Antigen
SKU: 20601 Categories: , ,
Overview
Product Name Anti-Rotavirus Group Antigen Antibody (20601)
Description Anti-Rotavirus A Mouse Monoclonal Antibody
Target Rotavirus Group Antigen
Species Reactivity Rotavirus Group Antigen
Applications WB,ELISA,IF
Host Mouse
Clonality Monoclonal
Clone ID 3C10
Isotype IgG2a
Immunogen Purified bovine rotavirus cell culture adapted strain Mr
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity These antibodies recognize bovine and human rotavirus A. #20601 also reacts with monkey rotavirus (SA-11) and porcine rotavirus (PP).
Target ID Rotavirus Group Antigen
Research Areas Infectious Disease
Application Images
Description Detection of RV strain SA11 in paraformaldehyde-fixed HT29 cells 9 hours post-infection. Cells were permeabilized with 0.1% Triton-X100 for 15 minutes at 4°C prior to staining.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 4°C or -20°C. If frozen, store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA, Immunoblotting, and IHC Immunofluorescence Detection of RV strain SA11 in paraformaldehyde-fixed HT29 cells 9 hours post-infection. Cells were permeabilized with 0.1% Triton-X100 for 15 minutes at 4°C prior to staining.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet