Reverse Triiodothyronine High Sensitivity ELISA Kit (ECP7936)
$790.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name Reverse Triiodothyronine High Sensitivity ELISA Kit (ECP7936)
Description Reverse Triiodothyronine ELISA Kit (ECP7936) is a Competitive Inhibition High Sensitivity ELISA for the quantitiation of Reverse Triiodothyronine in a variety of aqueous sample types. The high sensitivity kit offsets a reduced dynamic range for greater sensitivity compared to the standard kit.
Target Reverse Triiodothyronine
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 650.97 g·mol−1
Background Minimum Level of Detection: 9.04 pg/mL
Dynamic range of quantitation: 24.69-2,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 24.69-2,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Reverse Triiodothyronine Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Reverse Triiodothyronine Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 x 120uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| TMB Substrate | 1 x 9mL |
| Stop Solution | 1 x 6mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Reverse Triiodothyronine
Alternative Names 3,3',5'-Triiodo-L-thyronine, 5817-39-0, Reverse T3, 3,3',5'-Triiodothyronine, REVERSE TRIIODOTHYRONINE
CAS Number 5817-39-0
PubChem ID 644280
Research Areas Biochemicals
Background 3,3',5'-triiodo-L-thyronine is a 3,3',5'-triiodothyronine. It is a tautomer of a 3,3',5'-triiodo-L-thyronine zwitterion.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.