Recombinant Rat FGF-7 (Keratinocyte Growth Factor)
Catalog No.: 45102
Size: 20 ug
Source: Genetically-engineered E. coli. Molecular Weight: ~17,000 daltons Purity: >90%
Diluent: PBS/1.5 M NaCl
Purified by heparin affinity chroma- tography.
The activity of rat FGF-7 at 1-10 ng/ml is determined by tritiated thymidine incorporation using cultured mouse keratinocytes. Maximum stimulation observed at 1 ng/ml. The optimal concentration of this product for use with other cell types must be determined by the end user.
45102 binds to keratinocytes and most parenchymal epithelial cells; binds specific splice variant of FGF receptor expessed in epithelial cells (FGFr2IIIb).
Dilute with either 0.1% TFA/40% acetonitrile or PBS/0.1% CHAPS for best stability and to prevent absorption of product on walls of vessels.
STORAGE AND STABILITY
This product is stable for at least one (1) year at -20°C to -70°C. Keep frozen until use. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Yan, G. (1991) In Vitro Cell Dev Biol 27A: 437; Wang, F. et al (1995) J Biol Chem 270: 10222.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.