Alpha Crystallin B (11073MP-20)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 20ug | WB | Mouse |  |
Overview
Product Name Alpha Crystallin B (11073MP-20)
Description Recombinant Mouse CRYAB produced in E. coli is a single, non-glycosylated polypeptide chain containing 175 amino acids with a molecular weight of 20kDa.
Target Alpha Crystallin B
Species Reactivity Mouse
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution contains 20mM Tris buffer, pH 8.0 and 10% glycerol.
Buffer Formulation 20 mM Tris
Buffer pH pH 8.0
Buffer Cryopreservative 10% glycerol
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Background Alpha crystallins are composed of two gene products: alpha-A (acidic) and alpha-B (basic). Alpha crystallins are induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones. In addition, alpha crystallins have autokinase activity and play a role in intracellular architecture. Alpha crystallin B is expressed in many tissues and is associated with many neurological diseases.
Specificity Information
Target Name Alpha Crystallin B
Target ID Alpha Crystallin B
Alternative Names CRYAB Mouse
Sequence Location Cytoplasm, cytosol , Nucleus , Secreted , Lysosome , Note=Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. Localizes at the Z-bands and the intercalated disk in cardiomyocytes. Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion.
Sequence MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVA AAPKK
Biological Function May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (PubMed:31786107). {PubMed:31786107}.
Background Alpha crystallins are composed of two gene products: alpha-A (acidic) and alpha-B (basic). Alpha crystallins are induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones. In addition, alpha crystallins have autokinase activity and play a role in intracellular architecture. Alpha crystallin B is expressed in many tissues and is associated with many neurological diseases.
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below -20°C for longer periods of time. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet