Recombinant Human Vascular Endothelial Growth Factor (rHuVEGF)

Recombinant Human Vascular Endothelial Growth Factor

ORDERING INFORMATION

Format: Sterile filtered, lyophilized.
Purification: Chromatographic

DESCRIPTION

Recombinant Human VEGF produced in E. coli is a double, non-glycosylated, polypeptide chain containing 165 amino acids with a molecular mass of 38,231 daltons. This product has full biological activity.

SPECIFICATION SUMMARY

Source: Escherichia coli
Stabilizers: None
Preservatives: None
Purity: Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Dimers/Aggregates: <1% by SDS- PAGE
Biological Activity: The biological activity is determined by the dose- dependent stimulation of the proliferation of human umbilical vein endothelial cells using a concentration of 1.0 to 8.0 ng/ml.
Endotoxin: <0.1 ng/ug
Protein Content: Determined spectrophotometrically at 280nm (extinction coefficient 0.2875) and by RP-HPLC analysis against a calibrated standard.

SOLUBILITY

Reconstitute the lyophilized product in sterile water not less than 100 ug/ml.

STORAGE AND STABILITY

Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze- thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures