Recombinant Human Tumor Necrosis Factor – beta (rHuTNF-b)
Catalog No., Size:
50156P-5, 5 ug
50156P-20, 20 ug
Format: Sterile filtered, lyophilized.
Recombinant Human TNF-β produced in E. coli is a single, non-glycosated, polypeptide chain containing 171 amino acids with a molecular mass of 18.645 daltons. This product has full biological activity.
Source: Escherichia coli
Purity: Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Dimers/Aggregates: <1% by SDS-PAGE Biological Activity: Fully biologically active compared to reference standards. Specific Activity 2 x 10 7 IU/mg.
Endotoxin: <0.1 ng/ug Protein Content: Determined spectrophotometrically at 280nm (extinction coefficient 1.082) and by RP-HPLC analysis against a calibrated standard.
Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.