Recombinant Human Tumor Necrosis Factor – alpha (rHuTNF-a)

Recombinant Human Tumor Necrosis Factor – alpha (rHuTNF-a)

ORDERING INFORMATION

Catalog No., Size:
50154P-10, 10 ug
50154P-50, 50 ug
50154P-1000, 1000ug
Format: Sterile filtered, lyophilized.
Purification: Chromatographic

DESCRIPTION

Recombinant Human TNF-α produced in E. coli is a single, non-glycosated, polypeptide chain containing 158 amino acids with a molecular mass of 17,484 daltons. This product has full biological activity.

SPECIFICATION SUMMARY

Source: Escherichia coli
Preservatives: None
Purity: Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Dimers/Aggregates: <1% by SDS-PAGE Biological Activity: Fully biologically active compared to reference standards. Specific Activity 2 x 10 ⁷ IU/mg.
Endotoxin: <0.1 ng/ug Protein Content: Determined spectrophotometrically at 280nm (extinction coefficient 1.234) and by RP-HPLC analysis against a calibrated standard.

SOLUBILITY

Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.