Parathyroid Hormone (58004P-1000)
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 1000ug | WB | Human |  |
Overview
Product Name Parathyroid Hormone (58004P-1000)
Description Recombinant Human Parathyroid HormoneC181H290N55O51S2) is produced in E. coli as a single, non-glycosylated, polypeptide chain containing 34 amino acids and having a molecular mass of 4117.8 Dalton and is purified by proprietary chromatographic techniques.
Target Parathyroid Hormone
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Lyophilized
Formulation The protein (1 mg/ml) was lyophilized after extensive dialysis against 1.15mg sodium citrate, 7.31mg sodium chloride, 0.21 mg citric acid, 0.1117mg EDTA-Na2, 0.2mg Tween 80 and 50mg mannitol.
Format The protein (1 mg/ml) was lyophilized after extensive dialysis against 1.15mg sodium citrate, 7.31mg sodium chloride, 0.21 mg citric acid, 0.1117mg EDTA-Na2, 0.2mg Tween 80 and 50mg mannitol. Purity >98% as determined by RP-HPLC and SDS-PAGE.
Background Parathyroid hormone (PTH) is a 84-amino acid polypeptide secreted by parathyroid glands that increases the concentration of calcium in the blood through its interaction with parathyroid hormone receptors in bones, kidney, and intestines. In bones PTH enhances the release of calcium from the large reservoir contained in bones, in kidney it enhances active reabsorption of calcium from distal tubules and the thick ascending limb, and in intestines it enhances the absorption of calcium by increasing the production of vitamin D and upregulating the enzyme responsible for 1-alpha hydroxylation of 25-hydroxy vitamin D, converting vitamin D to its active form (1,25-dihydroxy vitamin D) which effects the absorption of calcium (as Ca2+ ions) by the intestine via calbindin.
Specificity Information
Target Name Parathyroid hormone
Target ID Parathyroid Hormone
Alternative Names rHuPTH
Sequence Ser-Val-Ser-Glu-Ile-Gln-Leu-Met-His-Asn-Leu-Gly-Lys-His-Leu-Asn-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Phe.
Biological Activity Parathyroid Hormone
Background Parathyroid hormone (PTH) is a 84-amino acid polypeptide secreted by parathyroid glands that increases the concentration of calcium in the blood through its interaction with parathyroid hormone receptors in bones, kidney, and intestines. In bones PTH enhances the release of calcium from the large reservoir contained in bones, in kidney it enhances active reabsorption of calcium from distal tubules and the thick ascending limb, and in intestines it enhances the absorption of calcium by increasing the production of vitamin D and upregulating the enzyme responsible for 1-alpha hydroxylation of 25-hydroxy vitamin D, converting vitamin D to its active form (1,25-dihydroxy vitamin D) which effects the absorption of calcium (as Ca2+ ions) by the intestine via calbindin.
Handling
Storage Lyophilized product should be stored desiccated below -20°C. Upon reconstitution, product may be stored at 4°C for 2-7 days and, for future use, below -20°C. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) is added. Prepare appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized rHuPTH in sterile distilled H2O to not less than 100μg/ml which can then be further diluted in other aqueous solutions.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet